Exercise #11: Bacterial Painting
In this exercise, you will learn use bacteria to create a work of art Learning Objectives At the end of the activity, you will be able...
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biocurio
Jul 19, 20222 min read
Exercise #10: Bacterial Transformations
In this exercise, you will learn how E. coli cells can be transformed by foreign plasmid DNA. The exercise uses frozen E. coli cells that...
2010
biocurio
Jul 19, 20221 min read
Exercise #9: Restriction Cloning
In this exercise, you will learn to perform a restriction cloning which will allow you to combine two elements from different plasmids...
460
biocurio
Jul 19, 20222 min read
Exercise #8: PCR Cleanup
In this exercise, you will perform a PCR Cleanup to remove extra material from your PCR reaction so that you can move forward with a pure...
210
biocurio
Jun 25, 20222 min read
Exercise #7: Polymerase Chain Reaction (PCR)
In this exercise, you will perform a polymerase chain reaction (PCR) to amplify the cloning region of a given series of cloning vectors....
650
biocurio
Jun 25, 20223 min read
Exercise #6: Running a Gel
Learning Objectives At the end of the activity, you will be able to: Abilities Run a gel electrophoresis and interpret the results....
410
biocurio
Jun 17, 20222 min read
Exercise #5: Diagnostic restriction analysis of plasmid DNA
In this exercise, you will learn to perform an in lab digest in order to learn how combining restriction endonuclease digestion is used...
860
biocurio
Jun 4, 20224 min read
Exercise 4: Mini-Preps
In this exercise, you will learn how to perform a mini-prep in order to extract and purify DNA from cell cultures, and how to use a...
760
biocurio
Jun 4, 20222 min read
Exercise 3: Visualizing and Annotative Plasmids
In this exercise, you will learn how to use the features on Benchling, such as annotating and visualizing a plasmid, as well as how to...
400
biocurio
Jun 2, 20224 min read
Exercise 2: Reviving E. Coli Cells from Long-Term Storage
In this exercise, you will learn how to obtain an isogenic E. coli cell culture from a frozen stock. This is important because storing...
580
biocurio
Jun 1, 20224 min read
Exercise 1: Preparing your Workbench
In this exercise, you will become familiar with the essential components and the preparation of a workbench for the manipulation of...
400
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